Serum non-coding RNAs as biomarkers for osteoarthritis progression after ACL injury.

ABSTRACT:

Objective

The aim of this study was to examine serum non-coding RNAs as potential biomarkers for cartilage damage associated with anterior cruciate ligament (ACL) injury.

Methods

Serum was obtained from 80 patients 1 year after surgery for ACL injury and 60 normal donors without overt skeletal injury. Total serum RNA was isolated, small non-coding RNAs profiled by TaqMan array MicroRNA (miRNA) analysis and individual small RNA assays performed by quantitative TaqMan RT-PCR (qPCR). Semi-quantitative magnetic resonance imaging (MRI) analysis was performed using Whole Organ Magnetic Resonance Knee Score (WORMS) scoring for analysis of cartilage damage.

Results

Initial TaqMan array miRNA profiling showed an increased serum concentration of a small nucleolar RNA (snoRNA),

SUBMITTER: Zhang L 

PROVIDER: S-EPMC3478481 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature