Easy and accurate calculation of programmed temperature gas chromatographic retention times by back-calculation of temperature and hold-up time profiles.

ABSTRACT: Linear retention indices are commonly used to identify compounds in programmed-temperature gas chromatography (GC), but they are unreliable unless the original experimental conditions used to measure them are stringently reproduced. However, differences in many experimental conditions may be properly taken into account by calculating programmed-temperature retention times of compounds from their measured isothermal retention vs. temperature relationships. We call this approach "retention projection". Until now, retention projection has been impractical because it required very precise, meticulous measurement of the temperature vs. time and hold-up time vs. temperature profiles actually produced by a specific GC instrument to be accurate. Here we present a new, easy-to-use methodology to pr

SUBMITTER: Boswell PG 

PROVIDER: S-EPMC3478941 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature