Characterization of bovine FUT7 furthers understanding of FUT7 evolution in mammals.

ABSTRACT:

Background

The Sialyl-Lewis X (Slex) is a well-known glycan structure involved in leukocyte homing and recruitment to inflammatory sites. SLex is well conserved among species and is mainly synthesized by FucT-VII in vertebrates. The enzyme responsible for its biosynthesis in cattle was not known.

Results

We cloned a cDNA sequence encoding bovine α3-fucosyltransferase VII that shares 83% identity with its human counterpart. Located at the BTA 11 telomeric region, the 1029 bp open reading frame is spread over two different exons, E1 which also contains the unique 5'-untranslated region and E2 which includes the entire 3'-untranslated region. The bfut7 expression pattern is restricted to thymus and spleen. A single transcript leading to the synthesis of a 342 aa protein was ide

SUBMITTER: Laporte B 

PROVIDER: S-EPMC3479001 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature