Rapid genetic targeting of pial surface neural progenitors and immature neurons by neonatal electroporation.

ABSTRACT:

Background

Recent findings have indicated the presence of a progenitor domain at the marginal zone/layer 1 of the cerebral cortex, and it has been suggested that these progenitors have neurogenic and gliogenic potential. However, their contribution to the histogenesis of the cortex remains poorly understood due to difficulties associated with genetically manipulating these unique cells in a population-specific manner.

Results

We have adapted the electroporation technique to target pial surface cells for rapid genetic manipulation at postnatal day 2. In vivo data show that most of these cells proliferate and progressively differentiate into both neuronal and glial subtypes. Furthermore, these cells localize to the superficial layers of the optic tectum and cerebral cortex pri

SUBMITTER: Breunig JJ 

PROVIDER: S-EPMC3479020 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature