Sp1 transcription factor and GATA1 cis-acting elements modulate testis-specific expression of mouse cyclin A1.

ABSTRACT: Cyclin A1 is a male germ cell-specific cell cycle regulator that is essential for spermatogenesis. It is unique among the cyclins by virtue of its highly restricted expression in vivo, being present in pachytene and diplotene spermatocytes and not in earlier or later stages of spermatogenesis. To begin to understand the molecular mechanisms responsible for this narrow window of expression of the mouse cyclin A1 (Ccna1) gene, we carried out a detailed analysis of its promoter. We defined a 170-bp region within the promoter and showed that it is involved in repression of Ccna1 in cultured cells. Within this region we identified known cis-acting transcription factor binding sequences, including an Sp1-binding site and two GATA1-binding sites. Neither Sp1 nor GATA1 is expressed in pachytene sp

SUBMITTER: Panigrahi SK 

PROVIDER: S-EPMC3480434 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature