Lactic acid is elevated in idiopathic pulmonary fibrosis and induces myofibroblast differentiation via pH-dependent activation of transforming growth factor-β.

ABSTRACT:

Rationale

Idiopathic pulmonary fibrosis (IPF) is a complex disease for which the pathogenesis is poorly understood. In this study, we identified lactic acid as a metabolite that is elevated in the lung tissue of patients with IPF.

Objectives

This study examines the effect of lactic acid on myofibroblast differentiation and pulmonary fibrosis.

Methods

We used metabolomic analysis to examine cellular metabolism in lung tissue from patients with IPF and determined the effects of lactic acid and lactate dehydrogenase-5 (LDH5) overexpression on myofibroblast differentiation and transforming growth factor (TGF)-β activation in vitro.

Measurements and main results

Lactic acid concentrations from healthy and IPF lung tissue were determined by nuclear magnetic resonance

SUBMITTER: Kottmann RM 

PROVIDER: S-EPMC3480515 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature