Altered DNA methylation profile in idiopathic pulmonary fibrosis.

ABSTRACT:

Rationale

DNA methylation is an important epigenetic mechanism, which often occurs in response to environmental stimuli and is crucial in regulating gene expression. It is likely that epigenetic alterations contribute to pathogenesis in idiopathic pulmonary fibrosis (IPF).

Objectives

To determine the DNA methylation changes in IPF and their effects on gene expression.

Methods

Total DNA methylation and DNA methyltransferase expression were compared in IPF and normal control lung tissues. IPF and normal tissues were subjected to comparative analysis of genome-wide DNA methylation and RNA expression using DNA hybridization to the Illumina HumanMethylation27 BeadChip and RNA hybridization to Illumina HumanHT-12 BeadChip. Functional analyses of differentially expressed and

SUBMITTER: Sanders YY 

PROVIDER: S-EPMC3480526 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature