Identification of resistant carboxylesterase alleles in Culex pipiens complex via PCR-RFLP.

ABSTRACT:

Background

Carboxylesterase overproduction is a frequently observed resistance mechanism of insects to organophosphate insecticides. As a major transmitter of human diseases, mosquitoes in the Culex pipiens complex have evolved 13 carboxylesterase alleles (Ester) that confer organophosphate resistance. Six alleles, Ester(B1), Ester², Ester⁸, Ester⁹, Ester(B10), and Ester¹¹, have been observed in field populations in China, sometimes co-existing in one population. To differentiate the carboxylesterase alleles found in these field populations, PCR-RFLP was designed for use in resistance monitoring.

Results

Based on the DNA sequences of resistant and nonresistant carboxylesterase alleles, Ester B alleles were first amplified with PCR-specific primers and then digested with the

SUBMITTER: Zhang H 

PROVIDER: S-EPMC3480951 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature