Innate immune regulation of Serratia marcescens-induced corneal inflammation and infection.

ABSTRACT:

Purpose

Serratia marcescens is frequently isolated from lenses of patients with contact lens-associated corneal infiltrates. In the current study, we examined the role of toll-like receptors (TLRs) and interleukin-1 receptor type 1 (IL-1R1) in S. marcescens-induced corneal inflammation and infection.

Methods

The central corneal epithelium of C57BL/6 and gene knockout mice was abraded, and 1 × 10(7) S. marcescens were added in the presence of a silicone hydrogel contact lens, and we examined corneal inflammation by confocal microscopy and neutrophil enumeration. Viable bacteria were quantified by colony-forming units (CFU).

Results

S. marcescens induced neutrophil recruitment to the corneal stroma, and increased corneal thickness and haze in C57BL/6 mice. Conversely, C

SUBMITTER: Zhou R 

PROVIDER: S-EPMC3481604 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature