The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells.

ABSTRACT: DNA transposons have become important vectors for efficient non-viral integration of transgenes into genomic DNA. The Sleeping Beauty (SB), piggyBac (PB), and Tol2 transposable elements have distinct biological properties and currently represent the most promising transposon systems for animal transgenesis and gene therapy. A potential obstacle, however, for persistent function of integrating vectors is transcriptional repression of the element and its genetic cargo. In this study we analyze the insulating effect of the 1.2-kb 5'-HS4 chicken β-globin (cHS4) insulator element in the context of SB, PB, and Tol2 transposon vectors. By examining transgene expression from genomically inserted transposon vectors encoding a marker gene driven by a silencing-prone promoter, we detect variable leve

SUBMITTER: Sharma N 

PROVIDER: S-EPMC3482222 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature