Chronic ethanol feeding alters miRNA expression dynamics during liver regeneration.

ABSTRACT:

Background

Adaptation to chronic ethanol (EtOH) treatment of rats results in a changed functional state of the liver and greatly inhibits its regenerative ability, which may contribute to the progression of alcoholic liver disease.

Methods

In this study, we investigated the effect of chronic EtOH intake on hepatic microRNA (miRNA) expression in male Sprague-Dawley rats during the initial 24 hours of liver regeneration following 70% partial hepatectomy (PHx) using miRNA microarrays. miRNA expression during adaptation to EtOH was investigated using RT-qPCR. Nuclear factor kappa B (NFκB) binding at target miRNA promoters was investigated with chromatin immunoprecipitation.

Results

Unsupervised clustering of miRNA expression profiles suggested that miRNA expression was mo

SUBMITTER: Dippold RP 

PROVIDER: S-EPMC3482264 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature