Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification-Based Specific Hybridization (FLASH) PCR.

ABSTRACT:

Background

Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans.

Methods

We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7.

Result

PCR product of 336 bp was successfully amplified in a FLASH-PCR.

Conclusion

As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7.

SUBMITTER: Khatami F 

PROVIDER: S-EPMC3482334 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature