Scavenger receptor mediated endocytosis of silver nanoparticles into J774A.1 macrophages is heterogeneous.

ABSTRACT: We investigated the scavenger receptor mediated uptake and subsequent intracellular spatial distribution and clustering of 57.7 ± 6.9 nm diameter silver nanoparticles (zeta-potential = -28.4 mV) in the murine macrophage cell line J774A.1 through colorimetric imaging. The NPs exhibited an overall red-shift of the plasmon resonance wavelength in the cell ensemble as function of time and concentration, indicative of intracellular NP agglomeration. A detailed analysis of the NP clustering in individual cells revealed a strong phenotypic variability in the intracellular NP organization on the single cell level. Throughout the observation time of 24h cells containing non- or low-agglomerated NPs with a characteristic blue color coexisted with cells containing NPs with varying degrees of agglomer

SUBMITTER: Wang H 

PROVIDER: S-EPMC3482345 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature