Transforming microbial genotyping: a robotic pipeline for genotyping bacterial strains.

ABSTRACT: Microbial genotyping increasingly deals with large numbers of samples, and data are commonly evaluated by unstructured approaches, such as spread-sheets. The efficiency, reliability and throughput of genotyping would benefit from the automation of manual manipulations within the context of sophisticated data storage. We developed a medium- throughput genotyping pipeline for MultiLocus Sequence Typing (MLST) of bacterial pathogens. This pipeline was implemented through a combination of four automated liquid handling systems, a Laboratory Information Management System (LIMS) consisting of a variety of dedicated commercial operating systems and programs, including a Sample Management System, plus numerous Python scripts. All tubes and microwell racks were bar-coded and their locations and sta

SUBMITTER: O'Farrell B 

PROVIDER: S-EPMC3483277 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature