Biochemical and structural characterization of recombinant human serum transferrin from rice (Oryza sativa L.).

ABSTRACT: The Fe(3+) binding protein human serum transferrin (hTF) is well known for its role in cellular iron delivery via the transferrin receptor (TFR). A new application is the use of hTF as a therapy and targeted drug delivery system for a number of diseases. Recently, production of hTF in plants has been reported; such systems provide a relatively inexpensive, animal-free (eliminating potential contamination by animal pathogens) method to produce large amounts of recombinant proteins for such biopharmaceutical applications. Specifically, the production of Optiferrin (hTF produced in rice, Oryza sativa, from InVitria) has been shown to yield large amounts of functional protein for use in culture medium for cellular iron delivery to promote growth. In the present work we describe further purific

SUBMITTER: Steere AN 

PROVIDER: S-EPMC3483368 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature