Measurement of S-nitrosylation occupancy in the myocardium with cysteine-reactive tandem mass tags: short communication.

ABSTRACT:

Rationale

S-nitrosylation (SNO) is a reversible, thiol-based protein modification that plays an important role in the myocardium by protecting critical cysteine residues from oxidation. However, little is known with regard to the percentage of a given protein that is modified by SNO (ie, SNO occupancy). Current methods allow for the relative quantification of SNO levels, but not for the determination of SNO occupancy.

Objective

To develop a method for the measurement of SNO occupancy, and apply this methodology to determine SNO occupancy in the myocardium.

Methods and results

We developed a differential cysteinereactive tandem mass tag (cysTMT) labeling procedure for the measurement of SNO occupancy. To validate this cysTMT labeling method, we treated whole-heart homo

SUBMITTER: Kohr MJ 

PROVIDER: S-EPMC3483371 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature