Synchrotron-based micro-CT imaging of the human lung acinus.

ABSTRACT: Structural data about the human lung fine structure are mainly based on stereological methods applied to serial sections. As these methods utilize 2D images, which are often not contiguous, they suffer from inaccuracies which are overcome by analysis of 3D micro-CT images of the never-sectioned specimen. The purpose of our study was to generate a complete data set of the intact three-dimensional architecture of the human acinus using high-resolution synchrotron-based micro-CT (synMCT). A human lung was inflation-fixed by formaldehyde ventilation and then scanned in a 64-slice CT over its apex to base extent. Lung samples (8-mm diameter, 10-mm height, N = 12) were punched out, stained with osmium tetroxide, and scanned using synMCT at (4 μm)(3) voxel size. The lung functional unit (acinus,

SUBMITTER: Litzlbauer HD 

PROVIDER: S-EPMC3483640 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature