Defining a gene promoter methylation signature in sputum for lung cancer risk assessment.

ABSTRACT:

Purpose

To evaluate the methylation state of 31 genes in sputum as biomarkers in an expanded nested, case-control study from the Colorado cohort, and to assess the replication of results from the most promising genes in an independent case-control study of asymptomatic patients with stage I lung cancer from New Mexico.

Experimental design

Cases and controls from Colorado and New Mexico were interrogated for methylation of up to 31 genes using nested, methylation-specific PCR. Individual genes and methylation indices were used to assess the association between methylation and lung cancer with logistic regression modeling.

Results

Seventeen genes with ORs of 1.4 to 3.6 were identified and selected for replication in the New Mexico study. Overall, the direction of effect

SUBMITTER: Leng S 

PROVIDER: S-EPMC3483793 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature