Cellular and molecular effect of MEHP Involving LXRα in human fetal testis and ovary.

ABSTRACT:

Background

Phthalates have been shown to have reprotoxic effects in rodents and human during fetal life. Previous studies indicate that some members of the nuclear receptor (NR) superfamilly potentially mediate phthalate effects. This study aimed to assess if expression of these nuclear receptors are modulated in the response to MEHP exposure on the human fetal gonads in vitro.

Methodology/principal findings

Testes and ovaries from 7 to 12 gestational weeks human fetuses were exposed to 10(-4)M MEHP for 72 h in vitro. Transcriptional level of NRs and of downstream genes was then investigated using TLDA (TaqMan Low Density Array) and qPCR approaches. To determine whether somatic or germ cells of the testis are involved in the response to MEHP exposure, we developed a highly e

SUBMITTER: Muczynski V 

PROVIDER: S-EPMC3484128 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature