Identification of genes required for alternative oxidase production in the Neurospora crassa gene knockout library.

ABSTRACT: The alternative oxidase (AOX) of Neurospora crassa transfers electrons from ubiquinol to oxygen. The enzyme is not expressed under normal conditions. However, when the function of the standard electron transport chain is compromised, AOX is induced, providing cells with a means to continue respiration and growth. Induction of the enzyme represents a form of retrograde regulation because AOX is encoded by a nuclear gene that responds to signals produced from inefficiently functioning mitochondria. To identify genes required for AOX expression, we have screened the N. crassa gene knockout library for strains that are unable to grow in the presence of antimycin A, an inhibitor of complex III of the standard electron transport chain. From the 7800 strains containing knockouts of different gene

SUBMITTER: Nargang FE 

PROVIDER: S-EPMC3484665 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature