Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments.

ABSTRACT:

Background

Multiplex PCR has been successfully applied in many areas since it was first reported in 1988; however, it suffers from poor universality.

Results

A novel method called Universal Multiplex PCR (UM-PCR) was created, which simultaneously amplifies multiple target fragments from genomic DNA. The method has two steps. First, the universal adapter-F and universal adapter-R are connected to the forward primers and the reverse primers, respectively. Hairpin structures and cross dimers of five pairs of adapter-primers are detected. Second, UM-PCR amplification is implemented using a novel PCR procedure termed "Two Rounds Mode" (three and 28-32 cycles). The first round (the first three cycles) is named the "One by One Annealing Round". The second round (28-32 cycles) combi

SUBMITTER: Wen D 

PROVIDER: S-EPMC3485162 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature