Comparison of the kinetics of maturation of phagosomes containing apoptotic cells and IgG-opsonized particles.

ABSTRACT: Defective clearance of apoptotic cells has emerged as an important contributing factor to the pathogenesis of many diseases. Although many efforts have been made to understand the machinery involved in the recognition between phagocytes and potential targets, little is known about the intracellular transport of phagosomes containing apoptotic cells within mammalian cells. We have, therefore, performed a detailed study on the maturation of phagosomes containing apoptotic cells in a non-professional phagocytic cell line. This process was compared with the maturation of IgG-opsonized particles, which are internalized via the Fcγ-receptor (Fcγ-R), one of the best characterized phagocytic receptor, in the same cell line stably expressing the Fcγ-RIIA. By comparing markers from different stages

SUBMITTER: Viegas MS 

PROVIDER: S-EPMC3485219 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature