Comparative proteomic profiling of human bile reveals SSP411 as a novel biomarker of cholangiocarcinoma.

ABSTRACT:

Background

Cholangiocarcinoma (CC) is an intractable cancer, arising from biliary epithelial cells, which has a poor prognosis and is increasing in incidence. Early diagnosis of CC is essential as surgical resection remains the only effective therapy. The purpose of this study was to identify improved biomarkers to facilitate early diagnosis and prognostication in CC.

Methods

A comparative expression profile of human bile samples from patients with cholangitis and CC was constructed using a classic 2D/MS/MS strategy and the expression of selected proteins was confirmed by Western blotting. Immunohistochemistry was performed to determine the expression levels of selected candidate biomarkers in CC and matched normal tissues. Finally, spermatogenesis associated 20 (SSP411; als

SUBMITTER: Shen J 

PROVIDER: S-EPMC3485295 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature