Mutation frequency and spectrum of mutations vary at different chromosomal positions of Pseudomonas putida.

ABSTRACT: It is still an open question whether mutation rate can vary across the bacterial chromosome. In this study, the occurrence of mutations within the same mutational target sequences at different chromosomal locations of Pseudomonas putida was monitored. For that purpose we constructed two mutation detection systems, one for monitoring the occurrence of a broad spectrum of mutations and transposition of IS element IS1411 inactivating LacI repressor, and another for detecting 1-bp deletions. Our results revealed that both the mutation frequency and the spectrum of mutations vary at different chromosomal positions. We observed higher mutation frequencies when the direction of transcription of the mutational target gene was opposite to the direction of replisome movement in the chromosome and vi

SUBMITTER: Juurik T 

PROVIDER: S-EPMC3485313 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature