Phylogenetically novel LuxI/LuxR-type quorum sensing systems isolated using a metagenomic approach.

ABSTRACT: A great deal of research has been done to understand bacterial cell-to-cell signaling systems, but there is still a large gap in our current knowledge because the majority of microorganisms in natural environments do not have cultivated representatives. Metagenomics is one approach to identify novel quorum sensing (QS) systems from uncultured bacteria in environmental samples. In this study, fosmid metagenomic libraries were constructed from a forest soil and an activated sludge from a coke plant, and the target genes were detected using a green fluorescent protein (GFP)-based Escherichia coli biosensor strain whose fluorescence was screened by spectrophotometry. DNA sequence analysis revealed two pairs of new LuxI family N-acyl-L-homoserine lactone (AHL) synthases and LuxR family transcri

SUBMITTER: Nasuno E 

PROVIDER: S-EPMC3485958 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature