Microbiome diversity in the bronchial tracts of patients with chronic obstructive pulmonary disease.

ABSTRACT: Culture of bacteria from bronchial secretions in respiratory patients has low sensitivity and does not allow for complete assessment of microbial diversity across different bronchial compartments. In addition, a significant number of clinical studies are based on sputum samples, and it is not known to what extent they describe the real diversity of the mucosa. In order to identify previously unrecognized lower airway bacteria and to investigate the complexity and distribution of microbiota in patients with chronic obstructive pulmonary disease (COPD), we performed PCR amplification and pyrosequencing of the 16S rRNA gene in patients not showing signs or symptoms of infection. Four types of respiratory samples (sputum, bronchial aspirate, bronchoalveolar lavage, and bronchial mucosa) were t

SUBMITTER: Cabrera-Rubio R 

PROVIDER: S-EPMC3486223 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature