A novel component of the Rhodobacter sphaeroides Fla1 flagellum is essential for motor rotation.

ABSTRACT: Here we describe a novel component essential for flagellar rotation in Rhodobacter sphaeroides. This protein is encoded by motF (RSP_0067), the first gene of a predicted transcriptional unit which contains two hypothetical genes. Sequence analysis indicated that MotF is a bitopic membrane-spanning protein. Protease sensitivity assays and green fluorescent protein (GFP) fusions confirmed this prediction and allowed us to conclude that the C terminus of MotF is located in the periplasmic space. Wild-type cells expressing a functional GFP-MotF fusion show a single fluorescent focus per cell. The localization of this protein in different genetic backgrounds allowed us to determine that normal localization of MotF depends on the presence of FliL and MotB. Characterization of a ΔmotF pseudorever

SUBMITTER: Ramirez-Cabrera V 

PROVIDER: S-EPMC3486363 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature