Determinants of translation elongation speed and ribosomal profiling biases in mouse embryonic stem cells.

ABSTRACT: Ribosomal profiling is a promising approach with increasing popularity for studying translation. This approach enables monitoring the ribosomal density along genes at a resolution of single nucleotides.In this study, we focused on ribosomal density profiles of mouse embryonic stem cells. Our analysis suggests, for the first time, that even in mammals such as M. musculus the elongation speed is significantly and directly affected by determinants of the coding sequence such as: 1) the adaptation of codons to the tRNA pool; 2) the local mRNA folding of the coding sequence; 3) the local charge of amino acids encoded in the codon sequence. In addition, our analyses suggest that in general, the translation velocity of ribosomes is slower at the beginning of the coding sequence and tends to incre

SUBMITTER: Dana A 

PROVIDER: S-EPMC3486846 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature