Ontology highlight
ABSTRACT:
SUBMITTER: Sosa MX
PROVIDER: S-EPMC3486893 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
PLoS computational biology 20121025 10
We describe methods for rapid sequencing of the entire human mitochondrial genome (mtgenome), which involve long-range PCR for specific amplification of the mtgenome, pyrosequencing, quantitative mapping of sequence reads to identify sequence variants and heteroplasmy, as well as de novo sequence assembly. These methods have been used to study 40 publicly available HapMap samples of European (CEU) and African (YRI) ancestry to demonstrate a sequencing error rate <5.63×10(-4), nucleotide diversit ...[more]