Arachidonic acid depletion extends survival of cold-stored platelets by interfering with the [glycoprotein Ibα--14-3-3ζ] association.

ABSTRACT:

Background

Cold storage of platelets reduces bacterial growth and preserves their hemostatic properties better than current procedures do. However, storage at 0°C induces [14-3-3ζ-glycoprotein Ibα] association, 14-3-3ζ release from phospho-Bad, Bad activation and apoptosis.

Design and methods

We investigated whether arachidonic acid, which also binds 14-3-3ζ, contributes to coldinduced apoptosis.

Results

Cold storage activated P38-mitogen-activated protein kinase and released arachidonic acid, which accumulated due to cold inactivation of cyclooxygenase-1/thromboxane synthase. Accumulated arachidonic acid released 14-3-3ζ from phospho-Bad and decreased the mitochondrial membrane potential, which are steps in the induction of apoptosis. Addition of arachidonic acid did

SUBMITTER: van der Wal DE 

PROVIDER: S-EPMC3487552 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature