New ratiometric optical oxygen and pH dual sensors with three emission colors for measuring photosynthetic activity in Cyanobacteria.

ABSTRACT: Photosynthetic algae and cyanobacteria have been proposed for producing biofuels through a direct photoconversion process. To accelerate the efforts of discovering and screening microbes for biofuel production, sensitive and high throughput methods to measure photosynthetic activity need to be developed. Here we report the development of new ratiometric optical oxygen and pH dual sensors with three emission colors for measuring photosynthetic activities directly. The dual sensor system can measure oxygen (O(2)) generation and pH increase resulted from carbon dioxide (CO(2)) consumption simultaneously. The sensor was prepared by a copolymerization of three monomeric probes, an intra-reference probe (IRP) which does not respond to pH or O(2), a probe for pH sensing (pHS), and an O(2) probe f

SUBMITTER: Lu H 

PROVIDER: S-EPMC3487715 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature