Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.

ABSTRACT:

Background

IL-17 is an important cytokine signature of the TH differentiation pathway TH17. This T-cell subset is crucial in mediating autoimmune disease or antimicrobial immunity in animal models, but its presence and role in human disease remain to be completely characterized.

Objective

We set out to determine the frequency of TH17 cells in patients with cystic fibrosis (CF), a disease in which there is recurrent infection with known pathogens.

Methods

Explanted lungs from patients undergoing transplantation or organ donors (CF samples=18; non-CF, nonbronchiectatic samples=10) were collected. Hilar nodes and parenchymal lung tissue were processed and examined for TH17 signature by using immunofluorescence and quantitative real-time PCR. T cells were isolated and sti

SUBMITTER: Chan YR 

PROVIDER: S-EPMC3488163 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature