A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity.

ABSTRACT: Recent advances have demonstrated the use of RNA-based control devices to program sophisticated cellular functions; however, the efficiency with which these devices can be quantitatively tailored has limited their broader implementation in cellular networks. Here, we developed a high-efficiency, high-throughput and quantitative two-color fluorescence-activated cell sorting-based screening strategy to support the rapid generation of ribozyme-based control devices with user-specified regulatory activities. The high-efficiency of this screening strategy enabled the isolation of a single functional sequence from a library of over 10(6) variants within two sorting cycles. We demonstrated the versatility of our approach by screening large libraries generated from randomizing individual component

SUBMITTER: Liang JC 

PROVIDER: S-EPMC3488204 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature