Sesamin stimulates osteoblast differentiation through p38 and ERK1/2 MAPK signaling pathways.

ABSTRACT:

Background

Osteoporosis is a worldwide health problem predominantly affecting post-menopausal women. Therapies aimed at increasing bone mass in osteoporetic patients lag behind comparable investigation of therapeutic strategies focusing on the bone resorption process. Sesamin, a major lignan compound found in Sesamun indicum Linn., has a variety of pharmacological effects, though its activity on bone cell function is unclear. Herein we examine the effect of this lignan on osteoblast differentiation and function.

Method

Cell cytotoxicity and proliferative in hFOB1.19 were examined by MTT and alamar blue assay up to 96 h of treatment. Gene expression of COL1, ALP, BMP-2, Runx2, OC, RANKL and OPG were detected after 24 h of sesamin treatment. ALP activity was measured at day 7,

SUBMITTER: Wanachewin O 

PROVIDER: S-EPMC3488317 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature