Dynamics of microRNAs in bull spermatozoa.

ABSTRACT:

Background

MicroRNAs are small non-coding RNAs that regulate gene expression and thus play important roles in mammalian development. However, the comprehensive lists of microRNAs, as well as, molecular mechanisms by which microRNAs regulate gene expression during gamete and embryo development are poorly defined. The objectives of this study were to determine microRNAs in bull sperm and predict their functions.

Methods

To accomplish our objectives we isolated miRNAs from sperm of high and low fertility bulls, conducted microRNA microarray experiments and validated expression of a panel of microRNAs using real time RT-PCR. Bioinformatic approaches were carried out to identify regulated targets.

Results

We demonstrated that an abundance of microRNAs were present in bovin

SUBMITTER: Govindaraju A 

PROVIDER: S-EPMC3488333 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature