High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia.

ABSTRACT:

Background

To investigate the potential association between oral health and cognitive function, a pilot study was conducted to evaluate high throughput DNA sequencing of the V3 region of the 16S ribosomal RNA gene for determining the relative abundance of bacterial taxa in subgingival plaque from older adults with or without dementia.

Methods

Subgingival plaque samples were obtained from ten individuals at least 70 years old who participated in a study to assess oral health and cognitive function. DNA was isolated from the samples and a gene segment from the V3 portion of the 16S bacterial ribosomal RNA gene was amplified and sequenced using an Illumina HiSeq1000 DNA sequencer. Bacterial populations found in the subgingival plaque were identified and assessed with respect to

SUBMITTER: Cockburn AF 

PROVIDER: S-EPMC3488532 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature