The microRNA miR-181c controls microglia-mediated neuronal apoptosis by suppressing tumor necrosis factor.

ABSTRACT:

Background

Post-ischemic microglial activation may contribute to neuronal damage through the release of large amounts of pro-inflammatory cytokines and neurotoxic factors. The involvement of microRNAs (miRNAs) in the pathogenesis of disorders related to the brain and central nervous system has been previously studied, but it remains unknown whether the production of pro-inflammatory cytokines is regulated by miRNAs.

Methods

BV-2 and primary rat microglial cells were activated by exposure to oxygen-glucose deprivation (OGD). Global cerebral ischemia was induced using the four-vessel occlusion (4-VO) model in rats. Induction of pro-inflammatory and neurotoxic factors, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and nitric oxide (NO), were assessed by ELISA, imm

SUBMITTER: Zhang L 

PROVIDER: S-EPMC3488569 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature