Level of phosphohistone H3 among various types of human cancers.

ABSTRACT: Anti-phosphorylated histone H3 (pHH3) antibodies specifically detect the core protein histone H3 only when phosphorylated at serine 10 (Ser10) or serine 28 (Ser28). Measurement of pHH3 levels can be used for quantifying mitosis and the effectiveness of mitotic inhibitors in early drug development. However, data on the expression level of pHH3 (Ser10) and pHH3 (Ser28) among different cancers are limited. This study was designed to investigate the expression levels of pHH3 across different types of cancers, using uniform techniques and assay platforms in a single laboratory. Retrospective study. Single laboratory. Formalin-fixed, paraffin-embedded various human cancer specimens were provided by Mosaic Laboratories Tissue Bank. Using immunohistochemistry, pHH3 levels were measured using both

SUBMITTER: Sun A 

PROVIDER: S-EPMC3488750 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature