Quantitative characterization of temperature-independent and temperature-dependent protein-protein interactions in highly nonideal solutions.

ABSTRACT: The interaction among each of three dilute "tracer" proteins (bovine serum albumin, superoxide dismutase, and ovomucoid) at a concentration of 2 mg/mL and each of two "crowder" proteins (ovomucoid and BSA) at concentrations up to 100 mg/mL was characterized by analysis of dependence of the equilibrium gradients of both tracer and crowder upon the concentration of crowder. The equilibrium gradients of both crowder proteins were found to be independent of temperature over the range 5-37 °C. The equilibrium gradients of tracer BSA and ovomucoid in the complementary crowder species were likewise found to be independent of temperature over this range, indicating that interaction among these tracers and crowders is predominantly repulsive and essentially entirely entropic in nature. The equilibr

SUBMITTER: Fodeke AA 

PROVIDER: S-EPMC3488770 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature