Targeted genome editing across species using ZFNs and TALENs.

ABSTRACT: Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.

SUBMITTER: Wood AJ 

PROVIDER: S-EPMC3489282 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature