Effects on human transcriptome of mutated BRCA1 BRCT domain: a microarray study.

ABSTRACT:

Background

BRCA1 (breast cancer 1, early onset) missense mutations have been detected in familial breast and ovarian cancers, but the role of these variants in cancer predisposition is often difficult to ascertain. In this work, the molecular mechanisms affected in human cells by two BRCA1 missense variants, M1775R and A1789T, both located in the second BRCT (BRCA1 C Terminus) domain, have been investigated. Both these variants were isolated from familial breast cancer patients and the study of their effect on yeast cell transcriptome has previously provided interesting clues to their possible role in the pathogenesis of breast cancer.

Methods

We compared by Human Whole Genome Microarrays the expression profiles of HeLa cells transfected with one or the other variant and HeL

SUBMITTER: Iofrida C 

PROVIDER: S-EPMC3489683 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature