Library preparation and multiplex capture for massive parallel sequencing applications made efficient and easy.

ABSTRACT: During the recent years, rapid development of sequencing technologies and a competitive market has enabled researchers to perform massive sequencing projects at a reasonable cost. As the price for the actual sequencing reactions drops, enabling more samples to be sequenced, the relative price for preparing libraries gets larger and the practical laboratory work becomes complex and tedious. We present a cost-effective strategy for simplified library preparation compatible with both whole genome- and targeted sequencing experiments. An optimized enzyme composition and reaction buffer reduces the number of required clean-up steps and allows for usage of bulk enzymes which makes the whole process cheap, efficient and simple. We also present a two-tagging strategy, which allows for multiplex se

SUBMITTER: Neiman M 

PROVIDER: S-EPMC3489721 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature