Neuron-to-neuron transmission of α-synuclein fibrils through axonal transport.

ABSTRACT:

Objective

The lesions of Parkinson disease spread through the brain in a characteristic pattern that corresponds to axonal projections. Previous observations suggest that misfolded α-synuclein could behave as a prion, moving from neuron to neuron and causing endogenous α-synuclein to misfold. Here, we characterized and quantified the axonal transport of α-synuclein fibrils and showed that fibrils could be transferred from axons to second-order neurons following anterograde transport.

Methods

We grew primary cortical mouse neurons in microfluidic devices to separate somata from axonal projections in fluidically isolated microenvironments. We used live-cell imaging and immunofluorescence to characterize the transport of fluorescent α-synuclein fibrils and their transfer to sec

SUBMITTER: Freundt EC 

PROVIDER: S-EPMC3490229 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature