DNA methylation changes between relapse and remission of minimal change nephrotic syndrome.

ABSTRACT:

Background

DNA methylation of gene promoters is associated with transcriptional inactivation. Changes in DNA methylation can lead to differences in gene expression levels and thereby influence disease development. We hypothesized that epigenetics underlies the pathogenesis of minimal change nephrotic syndrome (MCNS).

Methods

Genome-wide DNA methylation changes between relapse and remission in monocytes (n = 6) and naive T helper cells (Th0s) (n = 4) isolated from patients with MCNS were investigated using the microarray-based integrated analysis of methylation by isochizomers (MIAMI) method. We confirmed the MIAMI results using bisulfite-pyrosequencing analysis. Expression analysis was performed using quantitative real-time PCR.

Results

Three gene loci (GATA2, PBX4, a

SUBMITTER: Kobayashi Y 

PROVIDER: S-EPMC3491205 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature