Proteogenomic characterization and mapping of nucleosomes decoded by Brd and HP1 proteins.

ABSTRACT:

Background

Histone post-translational modifications (PTMs) constitute a branch of epigenetic mechanisms that can control the expression of eukaryotic genes in a heritable manner. Recent studies have identified several PTM-binding proteins containing diverse specialized domains whose recognition of specific PTM sites leads to gene activation or repression. Here, we present a high-throughput proteogenomic platform designed to characterize the nucleosomal make-up of chromatin enriched with a set of histone PTM binding proteins known as histone PTM readers. We support our findings with gene expression data correlating to PTM distribution.

Results

We isolated human mononucleosomes bound by the bromodomain-containing proteins Brd2, Brd3 and Brd4, and by the chromodomain-containing

SUBMITTER: LeRoy G 

PROVIDER: S-EPMC3491368 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature