Developmental features of DNA methylation during activation of the embryonic zebrafish genome.

ABSTRACT:

Background

Zygotic genome activation (ZGA) occurs at the mid-blastula transition (MBT) in zebrafish and is a period of extensive chromatin remodeling. Genome-scale gametic demethylation and remethylation occurs after fertilization, during blastula stages, but how ZGA relates to promoter DNA methylation states is unknown. Using methylated DNA immunoprecipitation coupled to high-density microarray hybridization, we characterize genome-wide promoter DNA methylation dynamics before, during and after ZGA onset, in relation to changes in post-translational histone modifications and gene expression.

Results

We show methylation of thousands of promoters before ZGA and additional methylation after ZGA, finding more dynamic methylation -1 to 0 kb upstream of the transcription start si

SUBMITTER: Andersen IS 

PROVIDER: S-EPMC3491385 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature