Chromatin accessibility reveals insights into androgen receptor activation and transcriptional specificity.

ABSTRACT:

Background

Epigenetic mechanisms such as chromatin accessibility impact transcription factor binding to DNA and transcriptional specificity. The androgen receptor (AR), a master regulator of the male phenotype and prostate cancer pathogenesis, acts primarily through ligand-activated transcription of target genes. Although several determinants of AR transcriptional specificity have been elucidated, our understanding of the interplay between chromatin accessibility and AR function remains incomplete.

Results

We used deep sequencing to assess chromatin structure via DNase I hypersensitivity and mRNA abundance, and paired these datasets with three independent AR ChIP-seq datasets. Our analysis revealed qualitative and quantitative differences in chromatin accessibility that corr

SUBMITTER: Tewari AK 

PROVIDER: S-EPMC3491416 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature