Genome-wide ribosome profiling reveals complex translational regulation in response to oxidative stress.

ABSTRACT: Information on unique and coordinated regulation of transcription and translation in response to stress is central to the understanding of cellular homeostasis. Here we used ribosome profiling coupled with next-generation sequencing to examine the interplay between transcription and translation under conditions of hydrogen peroxide treatment in Saccharomyces cerevisiae. Hydrogen peroxide treatment led to a massive and rapid increase in ribosome occupancy of short upstream ORFs, including those with non-AUG translational starts, and of the N-terminal regions of ORFs that preceded the transcriptional response. In addition, this treatment induced the synthesis of N-terminally extended proteins and elevated stop codon read-through and frameshift events. It also increased ribosome occupancy at

SUBMITTER: Gerashchenko MV 

PROVIDER: S-EPMC3491468 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature