Ontology highlight
ABSTRACT:
SUBMITTER: Lei Y
PROVIDER: S-EPMC3491516 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Lei Yong Y Guo Xiaogang X Liu Yun Y Cao Yang Y Deng Yi Y Chen Xiongfeng X Cheng Christopher H K CH Dawid Igor B IB Chen Yonglong Y Zhao Hui H
Proceedings of the National Academy of Sciences of the United States of America 20121008 43
Transcription activator-like effector nucleases (TALENs) are an approach for directed gene disruption and have been proved to be effective in various animal models. Here, we report that TALENs can induce somatic mutations in Xenopus embryos with reliably high efficiency and that such mutations are heritable through germ-line transmission. We modified the Golden Gate method for TALEN assembly to make the product suitable for RNA transcription and microinjection into Xenopus embryos. Eight pairs o ...[more]