Identification of antituberculosis agents that target ribosomal protein interactions using a yeast two-hybrid system.

ABSTRACT: Mycobacterium tuberculosis kills about 2 million people annually and antibiotic resistance is a cause of increased mortality. Therefore, development of new antituberculosis drugs is urgent for the control of widespread tuberculosis infections. For this purpose, we performed an innovative screen to identify new agents that disrupt the function of ribosomes in M. tuberculosis. Two bacterial ribosomal proteins L12 and L10 interact with each other and constitute the stalk of the 50S ribosomal subunit, which recruits initiation and elongation factors (EFs) during translation. Therefore, the L12-L10 interaction should be essential for ribosomal function and protein synthesis. We established a yeast two-hybrid system to identify small molecules that block the interaction between L12 and L10 prote

SUBMITTER: Lin Y 

PROVIDER: S-EPMC3491523 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature